Application of random amplified polymorphic DNA analysis in identifying Phellinus igniarius strains

Abstract

Described in this paper, the random amplified polymorphic DNA (RAPD) analysis was conducted with 20 random primers in various strains of Phellinus igniarius collected from different localities. The results showed that 17 of the 20 random primers were polymorphic ones. The DNA bands derived from each primer amplifying in tested strains ranged from 10 to 33. The size of the amplified DNA fragments ranged from 250 to 2000 base pairs. Of each test primer, a wide variation in banding profiles was observed among the 7 strains of P. igniarius. A total of 377 band positions were scored for all of the tested strains, which differed significantly among the bands from different primers. UPGMA cluster analysis subdivided the tested strains into two groups, which was helpful to find out the difference among the tested strains and to distinguish them directly.

Keywords

• phellinus igniarius
• rapd
• genetic relationship
• cluster analysis