Effect of pyrolysis temperature of biochar on Cd, Pb and As bioavailability and bacterial community composition in contaminated paddy soil

Abstract

To further investigate the effect of pyrolysis temperature on bioavailable Cd, Pb and As, as well as the bacterial community structure in multi-metal(loid) contaminated paddy soil, six types of biochar derived from wood sawdust and peanut shell at 300 °C, 500 °C and 700 °C were prepared and incubated with Cd, Pb and As contaminated paddy soil for 45 days. The results showed that adding biochar decreased bioavailable Cd by 31.3%− 42.9%, Pb by 0.61–56.1%, while bioavailable As changed from 9.68 mg kg−1 to 9.55–10.84 mg kg−1. We found that pyrolysis temperature of biochar had no significant effect on Cd bioavailability while Pb bioavailability decreased obviously with pyrolysis temperature raising. Biochar reduced the proportion of soluble and exchangeable Cd from 45.0% to 11.2–15.4% in comparison with the control, while no significant effect on the speciation of Pb and As. Wood sawdust biochar (WSBs) had more potential in decreasing bioavailable Cd and Pb than peanut shell biochar (PSBs). Although high-temperature biochar resulted a larger increase in bacterial species than low-and mid- temperature biochar, feedstock played a more important role in altering soil bacterial diversity and community composition than pyrolysis temperature. PSBs increased the diversity of soil bacteria through elevating soil dissolved carbon (DOC). Biochar altered soil bacterial community structure mainly by altering the level of soil electricity conductivity, DOC and bioavailable Cd. In addition, applying high-temperature PSBs increased the genus of bacteria that relevant to nitrogen cycling, such as Nitrospira, Nitrosotaleaceae and Candidatus_Nitrosotalea.

Keywords

• Heavy metal
• Environment
• Pollution
• Feedstock type
• Microbial community