Impact of 30 days exposure of whole Paper mill effluent (WRPBILE) on nucleic acid profile in the liver and gonad of freshwater teleost Mystus vittatus during annual reproductive cycle

Abstract

The present study has been undertaken to investigate the biochemical alterations in teleost fish Mystus vittatus after chronic exposure to sublethal concentrations of paper mill effluent for 30 days. a quantitative estimation of DNA and RNA material was made in liver and gonadal tissues throughout the reproductive cycle of the fish. The biochemical variables studied in the control fish showed the maximum values during the spawning phase as compared to other phases of the reproductive cycle of the fish. The changes produced in the nucleic acid content on account of chronic exposure of the fishes for 30 days to 0.4 (40%) and 0.8(80%) of 96 h LC50 of WRBBILE stress were found to be close dependent, being relatively much higher in case of 0.8 WRPBILE when compared to 0.4 WRPBILE. This phenomenon was observed during the three phases of the annual reproductive cycle of the fish. The DNA as well as RNA contents in liver, testis and ovary tissues showed a reduction in case of both the sublethal concentration of effluent in all the three phases of the reproductive cycle. The changes produced by WRPBILE stress were found to statistically very significant in all the phases except in the case of RNA content of testis during the post spawning phase of the fishes exposed to 0.4 WRPBILE. The present study concludes stress induced depletion might be due to degradation of cells, nuclear material and metabolic dysfunction in response to WRPBILE toxicity in the fish.

Keywords

• WRPBILE
• stress-induced
• spawning phase
• annual reproductive cycle
• chronic
• exposure