Study of Canine Distemper Virus Presence in Catalonia’s Wild Carnivores through H Gene Amplification and Sequencing

Abstract

Canine distemper virus (CDV) is recognised worldwide as an important pathogen in both domestic and wild carnivores. Few data are available on its impact and spread on the wildlife/wildlife–domestic animal–environment interface. This study, aimed at developing a conservation-oriented control strategy, analysed 89 sick or deceased animals from 2019 to 2023 at the Wildlife Rehabilitation Centre in Torreferrussa. RT-PCR and sequencing of the partial H gene were used to detect and analyse CDV in tissues. The total positive percentage was 20.22% (18/89), comprising 13 red foxes (44.8%), 4 European badgers (28.6%), and 1 American mink (4.5%), while 24 Eurasian otters tested negative. Phylogenetic analysis indicated that all of the CDV strains belong to the European lineage. Geographically distant individuals and different species shared the same viral strain, suggesting a strong capacity of CDV for interspecies and long-distance transmission. This calls for further research, particularly focusing on potential impacts of CDV on endangered carnivores.

Keywords

• red fox (<i>Vulpes vulpes</i>)
• badger (<i>Meles meles</i>)
• American mink (<i>Neogale vison</i>)
• Eurasian otter (<i>Lutra lutra</i>)
• canine distemper virus (CDV)