Resistance to Three Distinct Begomovirus Species in the Agronomical Superior Tropical Pumpkin Line AVPU1426 Developed at the World Vegetable Center
Narinder P. S. Dhillon,
Supornpun Srimat,
Suwannee Laenoi,
Anjana Bhunchoth,
Bencharong Phuangrat,
Nuchnard Warin,
Rungnapa Deeto,
Orawan Chatchawankanphanich,
Kriskamol Na Jom,
Sudathip Sae-tan,
Suk-Woo Jang,
Hyungjun Noh,
Roland Schafleitner,
Yuan-Li Chan,
Belén Picó,
Cristina Sáez,
Lawrence Kenyon
Affiliations
Narinder P. S. Dhillon
World Vegetable Center, East and Southeast Asia, Kasetsart University, Kamphaeng Saen 73140, Thailand
Supornpun Srimat
World Vegetable Center, East and Southeast Asia, Kasetsart University, Kamphaeng Saen 73140, Thailand
Suwannee Laenoi
World Vegetable Center, East and Southeast Asia, Kasetsart University, Kamphaeng Saen 73140, Thailand
Anjana Bhunchoth
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, Thailand
Bencharong Phuangrat
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, Thailand
Nuchnard Warin
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, Thailand
Rungnapa Deeto
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, Thailand
Orawan Chatchawankanphanich
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang 12120, Thailand
Kriskamol Na Jom
Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
Sudathip Sae-tan
Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
Suk-Woo Jang
National Institute of Horticultural & Herbal Science, Rural Development Administration, 370, Nongsaengmyeong-ro, Deockjin-gu, Jeonju-si 54874, Korea
Hyungjun Noh
National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, 370, Nongsaengmyeong-ro, Deockjin-gu, Jeonju-si 54874, Korea
Roland Schafleitner
Biotechnology, World Vegetable Center, 60 Yi-Min Liao, Shanhua, Tainan 74151, Taiwan
Yuan-Li Chan
Virology, World Vegetable Center, 60 Yi-Min Liao, Shanhua, Tainan 74151, Taiwan
Belén Picó
Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València (COMAV-UPV), 46022 València, Spain
Cristina Sáez
Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València (COMAV-UPV), 46022 València, Spain
Lawrence Kenyon
Virology, World Vegetable Center, 60 Yi-Min Liao, Shanhua, Tainan 74151, Taiwan
The Squash Leaf Curl China Virus (SLCCNV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) are species of Begomovirus (whitefly-vectored Geminiviridae) and cause serious damage to the cucurbit crops of the genus Cucurbita in the areas of South and Southeast Asia, across Asia, the Middle East and the Mediterranean, respectively. Cucurbita moschata’s inbred line AVPU1426, developed at the World Vegetable Center (WorldVeg) from a Bangladeshi landrace through pedigree selection, was observed to be resistant to both begomoviruses in field tests conducted at the WorldVeg Research and Training Station, Kasetsart University, Kamphaeng Saen, Thailand, which is a hotspot for these viruses. When AVPU1426 was tested for reaction to inoculation by viruliferous whiteflies with Squash Leaf Curl Philippines Virus (Taiwan strain) (SLCPV-TW) in the screen net-house at WorldVeg headquarters in Taiwan, it showed good resistance, though SLCPV-TW DNA-A could be detected in all inoculated plants, indicating that it did not show immunity to this virus. The objective of this study was to validate the resistance to SLCCNV and ToLCNDV in AVPU1426 by using whitefly-mediated inoculations to determine the mode of inheritance of the resistance. The results showed that AVPU1426 was resistant to SLCCNV and ToLCNDV. Upon crossing AVPU1426 with a susceptible check variety, Waltham Butternut, the resistance to both begomoviruses was observed to be conferred by a single recessive gene. This open-pollinated pumpkin line AVPU1426 bears flat round, mottled green immature fruits with yellow flesh in mature fruit. The fruit yield of AVPU1426 (20.74 t/ha−1) was comparable to ‘Rajah’ (18.61 t/ha−1), a recently released commercial F1 hybrid of East-West Seed (EWS). The fruit of AVPU1426 were estimated to have a good average β-carotene content (1.57 mg/100 g fresh weight). This line is a good source to breed pumpkins resistant to the three begomoviruses.
