Biotechnologie, Agronomie, Société et Environnement (2007-01-01)

Chromatographic, Spectrometric and NMR Characterization of a New Set of Glucuronic Acid Esters Synthesized by Lipase

  • Michel Marlier,
  • Michel Paquot,
  • Patrick Rollin,
  • Florence Chéry,
  • Jacqueline Destain,
  • Pascal Gerbaux,
  • Georges Lognay,
  • Christophe Blecker,
  • Benoît Moreau

Journal volume & issue
Vol. 11, no. 1
pp. 9 – 17

Abstract

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An enzymatic synthesis was developed on a new set of D-glucuronic acid esters and particularly the tetradecyl-D-glucopyranosiduronate also named tetradecyl D-glucuronate. Chromatographic analyses revealed the presence of the ester as a mixture of anomeric forms for carbon chain lengths superior to 12. TOF/MS and MS/MS studies confirmed the synthesis of glucuronic acid ester. The NMR study also confirmed the structure of glucuronic acid esters and clearly revealed an anomeric (α/β) ratio equivalent to 3/2

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