Neural Regeneration Research (Jan 2015)
Neuroprotective effect of rapamycin on spinal cord injury via activation of the Wnt/β-catenin signaling pathway
- Kai Gao,
- Yan-song Wang,
- Ya-jiang Yuan,
- Zhang-hui Wan,
- Tian-chen Yao,
- Hai-hong Li,
- Pei-fu Tang,
- Xi-fan Mei
Affiliations
- Kai Gao
- Yan-song Wang
- Ya-jiang Yuan
- Zhang-hui Wan
- Tian-chen Yao
- Hai-hong Li
- Pei-fu Tang
- Xi-fan Mei
- DOI
- https://doi.org/10.4103/1673-5374.158360
- Journal volume & issue
-
Vol. 10,
no. 6
pp. 951 – 957
Abstract
The Wnt/β-catenin signaling pathway plays a crucial role in neural development, axonal guidance, neuropathic pain remission and neuronal survival. In this study, we initially examined the effect of rapamycin on the Wnt/β-catenin signaling pathway after spinal cord injury, by intraperitoneally injecting spinal cord injured rats with rapamycin over 2 days. Western blot analysis and immunofluorescence staining were used to detect the expression levels of β-catenin protein, ca-spase-3 protein and brain-derived neurotrophic factor protein, components of the Wnt/β-catenin signaling pathway. Rapamycin increased the levels of β-catenin and brain-derived neurotrophic factor in the injured spinal cord, improved the pathological morphology at the injury site, reduced the loss of motor neurons, and promoted motor functional recovery in rats after spinal cord injury. Our experimental findings suggest that the neuroprotective effect of rapamycin intervention is mediated through activation of the Wnt/β-catenin signaling pathway after spinal cord injury.
Keywords
- microtubule
- axon
- kinesin-5
- Eg5
- regeneration
- monastrol
- molecular motor protein
- aging
- neurodegenerative disorders
- telomere shortening
- MSCs
- cellular therapy
- traumatic brain injury
- spinal cord injuries
- dual diagnosis
- diagnosis
- complications
- rehabilitation
- post-concussion syndrome
- brain concussion
- blood brain barrier
- phage display
- peptide library
- nanocarrier
- targeting
- Schwann cells
- neurite outgrowth
- neuromuscular junction (NMJ)
- multiple sclerosis
- TGF-β/BMP-7/Smad signaling
- myogenic differentiation
- Trf3
- tumor suppression
- nerve regeneration
- bone marrow mesenchymal stem cells
- cerebral ischemia
- tail vein injection
- middle cerebral artery occlusion
- cell therapy
- neuroprotection
- nerve regeneration
- brain injury
- neuroimaging
- ferumoxytol
- superparamagnetic iron oxide particles
- human adipose-derived stem cells
- middle cerebral artery occlusion
- intracerebral injection
- magnetic resonance imaging
- enhanced susceptibility-weighted angiography image
- modified neurological severity scores
- rats
- Prussian blue staining
- neural regeneration
- neural regeneration
- non-invasive brain stimulation
- transcranial magnetic stimulation
- neurotrophic factor
- brain-derived neurotrophic factor
- neuroplasticity
- hippocampus
- aging
- cognitive function
- nerve regeneration
- curcumin
- neurons
- HIV-1 gp120 V3 loop
- plasticity
- HIV-associated neurocognitive disorders
- output/input curve
- long-term potentiation
- excitatory postsynaptic potential
- paired-pulse facilitation
- Ca 2+
- synaptosome
- NSFC grants
- neural regeneration
- nerve regeneration
- brain injury
- hydrogen sulfide
- cerebral ischemia/reperfusion injury
- P2X 7 receptor
- 2
- 3
- 5-triphenyl-2H-tetrazolium chloride staining
- animal model
- protection
- sodium hydrosulfide
- immunofluorescence
- middle cerebral artery occlusion
- NSFC grant
- neural regeneration
- nerve regeneration
- γ-aminobutyric acid
- glial fibrillary acidic protein
- glutamic acid decarboxylase
- neurotoxicity
- weaning
- organ index
- cerebrum
- cortex
- glutamate
- neural regeneration
- p53 tumor suppressor gene family
- cerebral ischemia/reperfusion
- pyramidal neurons
- CA1 region
- delayed neuronal death
- immunohistochemistry
- western blotting
- neural regeneration
- nerve regeneration
- spinal cord injury
- rapamycin
- Wnt/β-catenin signaling pathway
- apoptosis
- caspase-3
- brain-derived neurotrophic factor
- neuroprotection
- loss of neurons
- NSFC grants
- neural regeneration