The <i>MyoD1</i> Promoted Muscle Differentiation and Generation by Activating <i>CCND2</i> in Guanling Cattle
Di Zhou,
Yan Wang,
Rong Yang,
Fu Wang,
Zhonghai Zhao,
Xin Wang,
Lingling Xie,
Xingzhou Tian,
Guoze Wang,
Bo Li,
Yu Gong
Affiliations
Di Zhou
Guizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, China
Yan Wang
Guizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, China
Rong Yang
Guizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, China
Fu Wang
Guizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, China
Zhonghai Zhao
Guizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, China
Xin Wang
Guizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, China
Lingling Xie
Guizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, China
Xingzhou Tian
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China
Guoze Wang
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China
Bo Li
Guizhou Livestock and Poultry Genetic Resources Management Station, Guiyang 550001, China
Yu Gong
Guizhou Livestock and Poultry Genetic Resources Management Station, Guiyang 550001, China
The purpose of this study was to analyze the transcriptome of MyoD1 gene knockout MDBK cells (bovine kidney cells) using high-throughput sequencing. For the first time, CRISPR/CAS9 technology was used to construct a MyoD1 knockout in MDBK cells and transcriptome sequence analysis was used to examine MyoD1-related target gene expression. Transcriptome sequencing indicated the presence of 723 differentially expressed genes (DEGs) by comparing wild type and MyoD1 knockout MDBK cells and included 178 upregulated and 72 downregulated genes. The DEGs are mainly enriched in Pl-3-kinase and AKT, p53 signaling pathways. Quantitative RT-PCR confirmed that PDE1B, ADAMTS1, DPT, and CCND2 were highly expressed in the leg muscle, longissimus dorsi, and shoulder of Guanling cattle, and CCND2 was inhibited after MyoD1 knockout, suggesting it may be a key downstream gene of MyoD1 and associated with muscle formation and differentiation in Guanling cattle. This provides experimental data for subsequent studies on the regulatory mechanisms of muscle differentiation in Guanling cattle.
