Frontiers in Microbiology (Mar 2023)
A food poisoning caused by ST7 Staphylococcal aureus harboring sea gene in Hainan province, China
- Yahui Guo,
- Yahui Guo,
- Yahui Guo,
- Xiaojie Yu,
- Jixiao Wang,
- De Hua,
- Yuanhai You,
- Qingbo Wu,
- Qinglong Ji,
- Jianzhong Zhang,
- Liefei Li,
- Yuan Hu,
- Zhonghui Wu,
- Xiaoyue Wei,
- Lianqun Jin,
- Fanliang Meng,
- Yuhua Yang,
- Xiaofeng Hu,
- Lijin Long,
- Songnian Hu,
- Heyuan Qi,
- Juncai Ma,
- Juncai Ma,
- Wenwen Bei,
- Xiaomei Yan,
- Haibin Wang,
- Haibin Wang,
- Zilong He
Affiliations
- Yahui Guo
- Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, China
- Yahui Guo
- Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China
- Yahui Guo
- Beijing Chaoyang District Center for Disease Control and Prevention, Beijing, China
- Xiaojie Yu
- Hainan Provincial Center for Disease Control and Prevention, Haikou, China
- Jixiao Wang
- Hainan Provincial Center for Disease Control and Prevention, Haikou, China
- De Hua
- Hainan Provincial Center for Disease Control and Prevention, Haikou, China
- Yuanhai You
- Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China
- Qingbo Wu
- Hainan Provincial Center for Disease Control and Prevention, Haikou, China
- Qinglong Ji
- Chinese Academy of Inspection and Quarantine, Beijing, China
- Jianzhong Zhang
- Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China
- Liefei Li
- Hainan Provincial Center for Disease Control and Prevention, Haikou, China
- Yuan Hu
- Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China
- Zhonghui Wu
- Hainan Provincial Center for Disease Control and Prevention, Haikou, China
- Xiaoyue Wei
- Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China
- Lianqun Jin
- Chinese PLA Center for Disease Control and Prevention, Beijing, China
- Fanliang Meng
- Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China
- Yuhua Yang
- Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China
- Xiaofeng Hu
- Chinese PLA Center for Disease Control and Prevention, Beijing, China
- Lijin Long
- Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China
- Songnian Hu
- State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
- Heyuan Qi
- Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
- Juncai Ma
- State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
- Juncai Ma
- Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
- Wenwen Bei
- Hainan Provincial Center for Disease Control and Prevention, Haikou, China
- Xiaomei Yan
- Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China
- Haibin Wang
- Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, China
- Haibin Wang
- Beijing Chaoyang District Center for Disease Control and Prevention, Beijing, China
- Zilong He
- Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Interdisciplinary Innovation Institute of Medicine and Engineering, School of Engineering Medicine, Beihang University, Beijing, China
- DOI
- https://doi.org/10.3389/fmicb.2023.1110720
- Journal volume & issue
-
Vol. 14
Abstract
ST7 Staphylococcus aureus is highly prevalent in humans, pigs, as well as food in China; however, staphylococcal food poisoning (SFP) caused by this ST type has rarely been reported. On May 13, 2017, an SFP outbreak caused by ST7 S. aureus strains occurred in two campuses of a kindergarten in Hainan Province, China. We investigated the genomic characteristics and phylogenetic analysis of ST7 SFP strains combined with the 91 ST7 food-borne strains from 12 provinces in China by performing whole-genome sequencing (WGS). There was clear phylogenetic clustering of seven SFP isolates. Six antibiotic genes including blaZ, ANT (4′)-Ib, tetK, lnuA, norA, and lmrS were present in all SFP strains and also showed a higher prevalence rate in 91 food-borne strains. A multiple resistance plasmid pDC53285 was present in SFP strain DC53285. Among 27 enterotoxin genes, only sea and selx were found in all SFP strains. A ФSa3int prophage containing type A immune evasion cluster (sea, scn, sak, and chp) was identified in SFP strain. In conclusion, we concluded that this SFP event was caused by the contamination of cakes with ST7 S. aureus. This study indicated the potential risk of new emergencing ST7 clone for SFP.
Keywords
- staphylococcus food poisoning
- sequence type 7
- whole-genome sequencing
- enterotoxin A
- prophage
- plasmids
