Nature Communications (Jul 2024)

The mouse multi-organ proteome from infancy to adulthood

  • Qingwen Wang,
  • Xinwen Ding,
  • Zhixiao Xu,
  • Boqian Wang,
  • Aiting Wang,
  • Liping Wang,
  • Yi Ding,
  • Sunfengda Song,
  • Youming Chen,
  • Shuang Zhang,
  • Lai Jiang,
  • Xianting Ding

DOI
https://doi.org/10.1038/s41467-024-50183-6
Journal volume & issue
Vol. 15, no. 1
pp. 1 – 14

Abstract

Read online

Abstract The early-life organ development and maturation shape the fundamental blueprint for later-life phenotype. However, a multi-organ proteome atlas from infancy to adulthood is currently not available. Herein, we present a comprehensive proteomic analysis of ten mouse organs (brain, heart, lung, liver, kidney, spleen, stomach, intestine, muscle and skin) at three crucial developmental stages (1-, 4- and 8-weeks after birth) acquired using data-independent acquisition mass spectrometry. We detect and quantify 11,533 protein groups across the ten organs and obtain 115 age-related differentially expressed protein groups that are co-expressed in all organs from infancy to adulthood. We find that spliceosome proteins prevalently play crucial regulatory roles in the early-life development of multiple organs, and detect organ-specific expression patterns and sexual dimorphism. This multi-organ proteome atlas provides a fundamental resource for understanding the molecular mechanisms underlying early-life organ development and maturation.