Generation of male-sterile soybean lines with the CRISPR/Cas9 system
Xiao Chen,
Suxin Yang,
Yaohua Zhang,
Xiaobin Zhu,
Xinjing Yang,
Chunbao Zhang,
Haiyan Li,
Xianzhong Feng
Affiliations
Xiao Chen
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun 130102, Jilin, China; University of Chinese Academy of Sciences, Beijing 100049, China
Suxin Yang
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun 130102, Jilin, China
Yaohua Zhang
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun 130102, Jilin, China
Xiaobin Zhu
School of Life Science, Jilin Agricultural University, Changchun 130118, Jilin, China
Xinjing Yang
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun 130102, Jilin, China; University of Chinese Academy of Sciences, Beijing 100049, China
Chunbao Zhang
Jilin Academy of Agricultural Sciences, Changchun 130000, Jilin, China
Haiyan Li
School of Life Science, Jilin Agricultural University, Changchun 130118, Jilin, China
Xianzhong Feng
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun 130102, Jilin, China; Corresponding author.
Soybean [Glycine max (L.) Merr.] provides a rich source of plant protein and oil worldwide. The commercial use of transgenic technology in soybean has become a classical example of the application of biotechnology to crop improvement. Although genetically modified soybeans have achieved commercial success, hybrid soybean breeding is also a potential way to increase soybean yield. Soybean cytoplasmic male-sterile (CMS) lines have been used in three-line hybrid breeding systems, but their application to exploiting soybean heterosis has been limited by rare germplasm resource of sterile lines. The generation of various genetic diversity male-sterile soybean lines will help to overcome the shortcoming. In this study, we used targeted editing of AMS homologs in soybean by CRISPR/Cas9 technology for the first time to generate stable male-sterile lines. Targeted editing of GmAMS1 resulted in a male-sterile phenotype, while editing of GmAMS2 failed to produce male-sterile lines. GmAMS1 functions not only in the formation of the pollen wall but also in the controlling the degradation of the soybean tapetum. CRISPR/Cas9 technology could be used to rapidly produce stable male-sterile lines, providing new sterile-line materials for soybean hybrid breeding systems.
