Molecular Therapy: Nucleic Acids (Dec 2021)

11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography

  • Anastasia V. Ozerskaya,
  • Tatiana N. Zamay,
  • Olga S. Kolovskaya,
  • Nikolay A. Tokarev,
  • Kirill V. Belugin,
  • Natalia G. Chanchikova,
  • Oleg N. Badmaev,
  • Galina S. Zamay,
  • Irina A. Shchugoreva,
  • Roman V. Moryachkov,
  • Vladimir N. Zabluda,
  • Vladimir A. Khorzhevskii,
  • Nikolay Shepelevich,
  • Stanislav V. Gappoev,
  • Elena A. Karlova,
  • Anastasia S. Saveleva,
  • Alexander A. Volzhentsev,
  • Anna N. Blagodatova,
  • Kirill A. Lukyanenko,
  • Dmitry V. Veprintsev,
  • Tatyana E. Smolyarova,
  • Felix N. Tomilin,
  • Sergey S. Zamay,
  • Vladimir N. Silnikov,
  • Maxim V. Berezovski,
  • Anna S. Kichkailo

Journal volume & issue
Vol. 26
pp. 1159 – 1172

Abstract

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Identification of primary tumors and metastasis sites is an essential step in cancer diagnostics and the following treatment. Positron emission tomography-computed tomography (PET/CT) is one of the most reliable methods for scanning the whole organism for malignancies. In this work, we synthesized an 11C-labeled oligonucleotide primer and hybridized it to an anti-cancer DNA aptamer. The 11C-aptamer was applied for in vivo imaging of Ehrlich ascites carcinoma and its metastases in mice using PET/CT. The imaging experiments with the 11C-aptamer determined very small primary and secondary tumors of 3 mm2 and less. We also compared 11C imaging with the standard radiotracer, 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18F-FDG), and found better selectivity of the 11C-aptamer to metastatic lesions in the metabolically active organs than 18F-FDG. 11C radionuclide with an ultra-short (20.38 min) half-life is considered safest for PET/CT imaging and does not cause false-positive results in heart imaging. Its combination with aptamers gives us high-specificity and high-contrast imaging of cancer cells and can be applied for PET/CT-guided drug delivery in cancer therapies.

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