Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software

Matthias Kaiser; Florian Jug; Thomas Julou; Siddharth Deshpande; Thomas Pfohl; Olin K. Silander; Gene Myers; Erik van Nimwegen

doi:10.1038/s41467-017-02505-0

Nature Communications (Jan 2018)

Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software

Matthias Kaiser,
Florian Jug,
Thomas Julou,
Siddharth Deshpande,
Thomas Pfohl,
Olin K. Silander,
Gene Myers,
Erik van Nimwegen

Affiliations

Matthias Kaiser: Biozentrum, University of Basel and Swiss Institute of Bioinformatics
Florian Jug: Max Planck Institute of Molecular Cell Biology and Genetics
Thomas Julou: Biozentrum, University of Basel and Swiss Institute of Bioinformatics
Siddharth Deshpande: Department of Chemistry, University of Basel
Thomas Pfohl: Department of Chemistry, University of Basel
Olin K. Silander: Biozentrum, University of Basel and Swiss Institute of Bioinformatics
Gene Myers: Max Planck Institute of Molecular Cell Biology and Genetics
Erik van Nimwegen: Biozentrum, University of Basel and Swiss Institute of Bioinformatics

DOI: https://doi.org/10.1038/s41467-017-02505-0
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 16

Abstract

Read online

How gene regulatory pathways control cell fate decisions in single cells is not fully understood. Here the authors present an integrated dual-input microfluidic chip and a linked analysis software, enabling tracking of gene regulatory responses of single bacterial cells to changing conditions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal