Methylated Fatty Acids from Heartwood and Bark of Pinus sylvestris, Abies alba, Picea abies, and Larix decidua: Effect of Strong Acid Treatment
Mohamed Zidan Mohamed Salem,
Ramadan A. Nasser,
Aleš Zeidler,
Hosam O. Elansary,
Ibrahim M. Aref,
Martin Böhm,
Hayssam M. Ali,
Abdalla I. Ahmed
Affiliations
Mohamed Zidan Mohamed Salem
Forestry and Wood Technology Department, Faculty of Agriculture (El–Shatby), Alexandria University, Egypt; Egypt
Ramadan A. Nasser
Forestry and Wood Technology Department, Faculty of Agriculture (EL–Shatby), Alexandria University, Egypt; Range and Forestry Applied Research Unit, Plant Production Dept., Food and Agricultural Sciences Collage, King Saud University, Saudi Arabia; Egypt
Aleš Zeidler
Department of Wood Processing, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Prague, Czech Republic; Czech Republic
Hosam O. Elansary
Biodiversity Institute of Ontario, University of Guelph, Guelph, ON, Canada; Canada
Ibrahim M. Aref
Range and Forestry Applied Research Unit, Plant Production Dept., Food and Agricultural Sciences Collage, King Saud University, Saudi Arabia; Saudi Arabia
Martin Böhm
Department of Wood Products and Wood Constructions, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Prague, Czech Republic; Czech Republic
Hayssam M. Ali
Timber Trees Research Department, Sabahia Horticulture Research Station, Horticulture Research Institute, Agriculture Research Center; Alexandria, Egypt; Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; Saudi Arabia
Abdalla I. Ahmed
Range and Forestry Applied Research Unit, Plant Production Dept., Food and Agricultural Sciences Collage, King Saud University, Saudi Arabia; Saudi Arabia
Methylated fatty acid (FA) compounds in the heartwood and bark of some softwood species, specifically Pinus sylvestris, Abies alba, Picea abies, and Larix decidua, grown in the Czech Republic were evaluated. Strong H2SO4 was used for methylation of the lipids. The highest content of lipid was found in P. abies bark (40.132 mg/g o.d. sample), and the lowest content was in A. alba wood (11.027 mg/g o.d. sample). The highest concentration of FAs was observed in L. decidua bark. The highest percentages of FAs in wood of P. sylvestris were arachidic acid and oleic acid. In bark, the highest percentages of FAs were stearic acid, palmitic acid, and oleic acid. The FAs with the highest concentrations in A. alba wood were arachidic acid, palmitic acid, pentadecanoic acid, and margarinic, and those in bark were behenic acid, lignoceric acid, and arachidic acid. P. abies wood FAs showed arachidic acid, palmitic acid, and margarinic acid, and the bark contained lignoceric acid and arachidic acid. The FAs of L. decidua wood were arachidic acid, palmitic acid, and stearic acid, and in bark they were pentacosylic acid, docosahexaenoic acid (DHA), lignoceric acid, arachidic acid, and behenic acid. The lack of typically dominant unsaturated fatty acids (e.g. 18:1, 18:2), compared to literature values were attributed to the application of strong acid for the hydrolysis.