Taḥqīqāt-i ̒Ulūm-i Raftārī (Jan 2024)
The Effect of Eight Weeks of Combined Exercises on the Expression of HIF1, VEGF, UCP1 Genes, and the Body Composition of Overweight Elderly Men
Abstract
Aim and Background: Due to the complications caused by overload on the cardiovascular system and the increase of these problems during old age, nowadays, combined exercises are used to reduce these complications. The aim of this study was to investigate the effect of eight weeks of combined exercises on the expression of HIF1, VEGF, UCP1 genes and the body composition of overweight elderly men. Materials and Methods: In this research; 20 elderly men randomly assigned to combined exercise group (10 people) and control group (10 people) with age range 60.70 ± 3.78 (years), weight 91.35 ± 2.66 (kg), height (meters) 1.74 ± 0.337, body mass index (kg/m2) 29.68 ± 1.67, fat mass (percent) 27.61 ± 1.22, muscle mass (kg) 30.03 ± 1.34 and The maximum oxygen consumption (milliliters/kg/minute) was 31.94 ± 2.25, were present. mixed group; They did resistance training for eight weeks and 3 sessions per week. In order to measure HIF1 and VEGF gene expression in white blood cells and PGC1a plasma levels, blood sampling was done 48 hours before the start of the first training session and also 48 hours after the last training session, while all subjects were fasting. Real-time PCR method was used to measure HIF1, VEGF and UCP1 gene expression. In order to analyze the data, independent and dependent t-test was used at a significance level of 0.05, with SPSS version 22 software. Findings: The results of the present study showed that eight weeks of combined exercises caused a significant increase in the expression of HIF1, VEGF, UCP1 genes. There was a significant decrease in fat mass and body mass index (P=0.001). Conclusion: The findings of this research showed that combined exercises with the model described in this research should be used to improve the physiological condition and improve the body composition of elderly people
