Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers

Xiao Liu; Kevin Lam; Huayi Zhao; Sadatsugu Sakane; Hyun Young Kim; Alvaro Eguileor; Karin Diggle; Shuai Wu; Raquel Carvalho Gontijo Weber; Pejman Soroosh; Mojgan Hosseini; Kristin Mekeel; David A. Brenner; Tatiana Kisseleva

STAR Protocols (Sep 2023)

Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers

Xiao Liu,
Kevin Lam,
Huayi Zhao,
Sadatsugu Sakane,
Hyun Young Kim,
Alvaro Eguileor,
Karin Diggle,
Shuai Wu,
Raquel Carvalho Gontijo Weber,
Pejman Soroosh,
Mojgan Hosseini,
Kristin Mekeel,
David A. Brenner,
Tatiana Kisseleva

Affiliations

Xiao Liu: Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Corresponding author
Kevin Lam: Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
Huayi Zhao: Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
Sadatsugu Sakane: Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
Hyun Young Kim: Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
Alvaro Eguileor: Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
Karin Diggle: Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
Shuai Wu: State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen, China
Raquel Carvalho Gontijo Weber: Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
Pejman Soroosh: Janssen Pharmaceutical R&D, Immunometabolism Obesity and Metabolic Disorders, San Diego, CA, USA
Mojgan Hosseini: Department of Pathology, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
Kristin Mekeel: Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
David A. Brenner: Department of Medicine, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA
Tatiana Kisseleva: Department of Surgery, University of California, La Jolla, San Diego School of Medicine, La Jolla, CA, USA; Corresponding author

Journal volume & issue: Vol. 4, no. 3
p. 102391

Abstract

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Summary: Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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