Data on characterization and validation of assays for ultrasensitive quantitative detection of small molecules: Determination of free thyroxine with magnetic and interferometric methods
A.V. Orlov,
S.L. Znoyko,
A.V. Pushkarev,
E.N. Mochalova,
N.V. Guteneva,
A.V. Lunin,
M.P. Nikitin,
P.I. Nikitin
Affiliations
A.V. Orlov
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
S.L. Znoyko
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow 119991, Russia
A.V. Pushkarev
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
E.N. Mochalova
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
N.V. Guteneva
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
A.V. Lunin
Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
M.P. Nikitin
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow 119991, Russia; Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
P.I. Nikitin
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow 119991, Russia; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 31 Kashirskoe shosse, Moscow 115409, Russia; Corresponding author at: Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow 119991, Russia.
The presented data refer to optimization and quantitative characterization of a rapid lateral flow assay based on high-affinity bifunctional ligand and magnetic nanolabels, which was developed for detection of small molecules of thyroid hormones. The results were obtained by several techniques, including the magnetic particle quantification method, spectral-correlation interferometry and spectral-phase interferometry, dynamic light scattering, enzyme linked immunosorbent assay. The long-term stability of “antibody – magnetic nanoparticle” conjugates is shown. The assay specificity is confirmed, and verification of successful combination of magnetic particles and antibodies is demonstrated. The kinetic and equilibrium dissociation constants are determined for interactions between thyroxine and monoclonal antibodies. The obtained data could be used for design of other platforms for detection of small molecules.
