Generation of Human Stomach Cancer iPSC-Derived Organoids Induced by <i>Helicobacter pylori</i> Infection and Their Application to Gastric Cancer Research
Chia-Chen Ku,
Kenly Wuputra,
Jia-Bin Pan,
Chia-Pei Li,
Chung-Jung Liu,
Yi-Chang Liu,
Shigeo Saito,
Te-Fu Chan,
Chang-Shen Lin,
Deng-Chyang Wu,
Kazunari K. Yokoyama
Affiliations
Chia-Chen Ku
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Kenly Wuputra
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Jia-Bin Pan
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Chia-Pei Li
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Chung-Jung Liu
Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Yi-Chang Liu
Cell Therapy and Research Center, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan
Shigeo Saito
Waseda Research Institute of Science and Engineering, Waseda University, Tokyo 169-0051, Japan
Te-Fu Chan
Department of Obstetrics and Genecology, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan
Chang-Shen Lin
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Deng-Chyang Wu
Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Kazunari K. Yokoyama
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
There is considerable cellular diversity in the human stomach, which has helped to clarify cell plasticity in normal development and tumorigenesis. Thus, the stomach is an interesting model for understanding cellular plasticity and for developing prospective anticancer therapeutic agents. However, many questions remain regarding the development of cancers in vivo and in vitro in two- or three-dimensional (2D/3D) cultures, as well as the role of Helicobacter pylori (H. p.) infection. Here, we focus on the characteristics of cancer stem cells and their derived 3D organoids in culture, including the formation of stem cell niches. We define the conditions required for such organoid culture in vitro and examine the ability of such models for testing the use of anticancer agents. We also summarize the signaling cascades and the specific markers of stomach-cancer-derived organoids induced by H. p. infection, and their stem cell niches.
