Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples
Jialan Cao,
Felix Richter,
Michael Kastl,
Jonny Erdmann,
Christian Burgold,
David Dittrich,
Steffen Schneider,
J. Michael Köhler,
G. Alexander Groß
Affiliations
Jialan Cao
Institute for Chemistry and Biotechnologies, Department of Physical Chemistry and Microreaction Technologies, Technische Universität Ilmenau, 98693 Ilmenau, Germany
Felix Richter
Institute for Chemistry and Biotechnologies, Department of Physical Chemistry and Microreaction Technologies, Technische Universität Ilmenau, 98693 Ilmenau, Germany
Michael Kastl
CETONI GmbH Automatisierung und Microsysteme, 07554 Korbussen, Germany
Jonny Erdmann
CETONI GmbH Automatisierung und Microsysteme, 07554 Korbussen, Germany
Christian Burgold
CETONI GmbH Automatisierung und Microsysteme, 07554 Korbussen, Germany
David Dittrich
CETONI GmbH Automatisierung und Microsysteme, 07554 Korbussen, Germany
Steffen Schneider
Institute for Chemistry and Biotechnologies, Department of Physical Chemistry and Microreaction Technologies, Technische Universität Ilmenau, 98693 Ilmenau, Germany
J. Michael Köhler
Institute for Chemistry and Biotechnologies, Department of Physical Chemistry and Microreaction Technologies, Technische Universität Ilmenau, 98693 Ilmenau, Germany
G. Alexander Groß
Institute for Chemistry and Biotechnologies, Department of Physical Chemistry and Microreaction Technologies, Technische Universität Ilmenau, 98693 Ilmenau, Germany
Droplet-based microfluidics is a versatile tool to reveal the dose–response relationship of different effectors on the microbial proliferation. Traditional readout parameter is the temporal development of the cell density for different effector concentrations. To determine nonlinear or unconventional dose–response relationships, data with high temporal resolution and dense concentration graduation are essential. If microorganisms with slow microbial growth kinetics are investigated, a sterile and evaporation-free long-term incubation technique is required. Here, we present a modular droplet-based screening system which was developed to solve these issues. Beside relevant technical aspects of the developed modules, the procedural workflow, and exemplary dose–response data for 1D and 2D dose–response screenings are presented.
