Identification of the Inner Cell Mass and the Trophectoderm Responses after an In Vitro Exposure to Glucose and Insulin during the Preimplantation Period in the Rabbit Embryo
Romina Via y Rada,
Nathalie Daniel,
Catherine Archilla,
Anne Frambourg,
Luc Jouneau,
Yan Jaszczyszyn,
Gilles Charpigny,
Véronique Duranthon,
Sophie Calderari
Affiliations
Romina Via y Rada
BREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France
Nathalie Daniel
BREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France
Catherine Archilla
BREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France
Anne Frambourg
BREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France
Luc Jouneau
BREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France
Yan Jaszczyszyn
Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CNRS, CEA, Paris-Sud University F, 91190 Gif-sur-Yvette, France
Gilles Charpigny
BREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France
Véronique Duranthon
BREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France
Sophie Calderari
BREED INRAE, UVSQ, Université Paris-Saclay, 78350 Jouy-en-Josas, France
The prevalence of metabolic diseases is increasing, leading to more women entering pregnancy with alterations in the glucose-insulin axis. The aim of this work was to investigate the effect of a hyperglycemic and/or hyperinsulinemic environment on the development of the preimplantation embryo. In rabbit embryos developed in vitro in the presence of high insulin (HI), high glucose (HG), or both (HGI), we determined the transcriptomes of the inner cell mass (ICM) and the trophectoderm (TE). HI induced 10 differentially expressed genes (DEG) in ICM and 1 in TE. HG ICM exhibited 41 DEGs involved in oxidative phosphorylation (OXPHOS) and cell number regulation. In HG ICM, proliferation was decreased (p p p p p < 0.001). Exposure to HG and HGI during preimplantation development results in common and specific ICM and TE responses that could compromise the development of the future individual and placenta.
