Tauro-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard in the gas-liquid chromatographic analysis of bile acid methyl ester acetates.

Abstract

Tauro-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid has been used as internal standard in the gas-liquid chromatographic analysis of bile acid methyl ester acetates. The advantage of this compound over other internal standards is that its use takes into account the hydrolysis rate of the bile acids. The entire procedure is monitored by thin-layer chromatography, gas-liquid chromatography, and by radioactivity measurement and zonal scanning.