Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine
Ştefan Ciornei,
Dan Drugociu,
Liliana Margareta Ciornei,
Mihai Mareş,
Petru Roşca
Affiliations
Ştefan Ciornei
Reproduction Clinic, Department Clinics, Faculty of Veterinary Medicine, Iasi University of Life Sciences (IULS), M. Sadoveanu Alee, no 6, 700489 Iaşi, Romania
Dan Drugociu
Reproduction Clinic, Department Clinics, Faculty of Veterinary Medicine, Iasi University of Life Sciences (IULS), M. Sadoveanu Alee, no 6, 700489 Iaşi, Romania
Liliana Margareta Ciornei
Reproduction Clinic, Department Clinics, Faculty of Veterinary Medicine, Iasi University of Life Sciences (IULS), M. Sadoveanu Alee, no 6, 700489 Iaşi, Romania
Mihai Mareş
Laboratory of Microbiology, Department of Public Health, Faculty of Veterinary Medicine, Iasi University of Life Sciences (IULS), M. Sadoveanu Alee, no 6, 700489 Iaşi, Romania
Petru Roşca
Reproduction Clinic, Department Clinics, Faculty of Veterinary Medicine, Iasi University of Life Sciences (IULS), M. Sadoveanu Alee, no 6, 700489 Iaşi, Romania
The aim of the study was to establish the complete microbiological profile of boar semen (Sus scrofa domesticus) and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pig farms. One hundred and one semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by evaluating the degree of contamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149 × 103 CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 × 103 CFU/mL. Twenty-four germs (15 bacterial and 9 fungal species) were isolated, the most common being Candida parapsilosis/sake (92%) and Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm by 49.85% in bacteria (significant (p p p > 0.05).
