Expression of Reg3g after brain trauma and its effect on early epilepsy in mice

Abstract

Objective To investigate the temporal and spatial expression profile of Reg3g signal activation in traumatic brain injury (TBI) and its effect on early seizure sensitivity of TBI. Methods TBI model was established with controlled cortical impact (CCI).In molecular and morphological experiments, 60 C57BL/6N wild type (WT) mice were randomly divided into sham group, CCI 24-h group, CCI 72-h group and CCI 1-week group.The temporal and spatial expression of Reg3g was detected by RT-PCR, Western blotting and immunofluorescence assay.Epilepsy was induced by kainic acid (KA).In the brain trauma seizure experiment, 30 WT mice and 30 Reg3g KO mice were randomly divided into WT (CCI)+(normal saline, NS) group, Reg3g KO (CCI)+NS group, WT (CCI)+KA group and Reg3g KO (CCI)+KA group.Epileptic seizures were induced on the basis of CCI, and electroencephalography (EEG) was performed in vivo. Results ① Compared with the sham group, the mRNA and protein expression of Reg3g in the cortex of the CCI injured side was significantly increased in 24 and 72 h and 1 week after CCI (P < 0.01).② The results of immunofluorescence assay showed that Reg3g and NeuN were completely co-expressed in the neurons in the cortex of CCI mice.③ No obvious epileptic waves were observed in WT and Reg3g KO mice injected with NS.Significant epileptic waves were observed in WT mice and Reg3g KO mice injected with KA.Compared with the WT mice, the latency of Reg3g KO mice injected with KA was significantly shorter and the total attack time was significantly longer (P < 0.05). Conclusion Reg3g plays a significant protective role in early seizures after TBI, and it may be an important regulatory target to inhibit early seizures after TBI.

Keywords

• traumatic brain injury
• reg3g
• seizures
• sensitivity