Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3

Kazuto Yoshimi; Kohei Takeshita; Noriyuki Kodera; Satomi Shibumura; Yuko Yamauchi; Mine Omatsu; Kenichi Umeda; Yayoi Kunihiro; Masaki Yamamoto; Tomoji Mashimo

doi:10.1038/s41467-022-32618-0

Nature Communications (Aug 2022)

Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3

Kazuto Yoshimi,
Kohei Takeshita,
Noriyuki Kodera,
Satomi Shibumura,
Yuko Yamauchi,
Mine Omatsu,
Kenichi Umeda,
Yayoi Kunihiro,
Masaki Yamamoto,
Tomoji Mashimo

Affiliations

Kazuto Yoshimi: Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo
Kohei Takeshita: Life Science Research Infrastructure Group, Advanced Photon Technology Division
Noriyuki Kodera: Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi
Satomi Shibumura: C4U Corporation
Yuko Yamauchi: Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo
Mine Omatsu: Life Science Research Infrastructure Group, Advanced Photon Technology Division
Kenichi Umeda: Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi
Yayoi Kunihiro: C4U Corporation
Masaki Yamamoto: Life Science Research Infrastructure Group, Advanced Photon Technology Division
Tomoji Mashimo: Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo

DOI: https://doi.org/10.1038/s41467-022-32618-0
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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In this study, Yoshimi et al. report on the mechanisms of CRISPR-Cas3 non-specific cleavage of single-stranded DNA and target-specific degradation of double stranded DNA. They employ high-speed AFM to capture images of type I CRISPR priming and interference.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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