Protein analysis of rat hepatocytes under conditions glutamate-induced obesity and its correction

Abstract

We had characterized low-, medium- and high-molecular protein fractions of hepatocytes under development of glutamate-induced obesity and correction of nanocrystalline cerium dioxide and pioglitazone. Protein fractions were separated by electrophoresis using a 10 % Laemmli SDS-PAGE sodium dodecyl sulfate. Protein hepatocytes change under glutamate-induced obesity: high-protein reduced, and low-protein increased. Changes in hepatocyte proteins are consistent with previously established changes in protein content of hepatocytes under the influence HCD rich in fats and carbohydrates. We had noticed similar changes in protein of hepatocytes under correction cerium dioxide, but compared with glutamate-induced obesity, low proteins were lower. Pioglitazone didn't show a positive effect on hepatocyte proteins that may be associated with short-term administration.

Keywords

• glutamate-induced obesity, cerium dioxide, pioglitazone, protein fractions, hepatocytes