Applications of optical DNA mapping in microbiology
Diana Bogas,
Lena Nyberg,
Rui Pacheco,
Nuno F. Azevedo,
Jason P. Beech,
Margarita Gomila,
Jorge Lalucat,
Célia M. Manaia,
Olga C. Nunes,
Jonas O. Tegenfeldt,
Fredrik Westerlund
Affiliations
Diana Bogas
1Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal
Lena Nyberg
2Chemical Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
Rui Pacheco
1Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal
Nuno F. Azevedo
3LEPABE – Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculdade de Engenharia, Universidade do Porto, Porto, Portugal
Jason P. Beech
4NanoLund and Department of Physics, Lund University, Lund, Sweden
Margarita Gomila
5Microbiology, Biology Department, Universitat de les Illes Balears, Palma de Mallorca, Balearic Islands, Spain
Jorge Lalucat
5Microbiology, Biology Department, Universitat de les Illes Balears, Palma de Mallorca, Balearic Islands, Spain
Célia M. Manaia
1Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal
Olga C. Nunes
3LEPABE – Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculdade de Engenharia, Universidade do Porto, Porto, Portugal
Jonas O. Tegenfeldt
4NanoLund and Department of Physics, Lund University, Lund, Sweden
Fredrik Westerlund
2Chemical Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence–based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.
