Cancer Management and Research (2020-11-01)

Baohuoside I via mTOR Apoptotic Signaling to Inhibit Glioma Cell Growth

  • Guo Y,
  • Wang C,
  • Jiang M,
  • Zhu H,
  • Weng M,
  • Sun L,
  • Zhang Y

Journal volume & issue
Vol. Volume 12
pp. 11435 – 11444

Abstract

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Yangyang Guo,1,* Cheng Wang,1,* Minghui Jiang,1 Hengyue Zhu,1 Min Weng,1 Linxiao Sun,1 Yanlei Zhang2 1Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, Zhejiang Provincial Top Key Discipline in Surgery, Wenzhou Medical University First Affiliated Hospital, Wenzhou, Zhejiang, People’s Republic of China; 2Neurology Department, Wenzhou Medical University First Affiliated Hospital, Wenzhou, Zhejiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yanlei Zhang; Linxiao Sun Email [email protected]; [email protected]: Baohuoside I, a novel oncotherapeutic agent, has been reported to have anti-cancer effects on a variety of cancers, but its role in glioma and its molecular mechanism are still unclear.Methods: The proliferation of U251 cells was detected by real-time cellular analysis (RTCA), CCK-8, Ki67 immunofluorescence and colony formation assay. The effect of Baohuoside I on the invasion and migration of U251 cells was measured by transwell and scratch tests. The apoptosis of U251 cells was detected by flow cytometry. The expression level of related protein was detected by western blotting.Results: Baohuoside I could inhibit the proliferation of human glioma cells and induce apoptosis. Further study showed that the migration and invasion ability of glioma was significantly decreased by Baohuoside I. Western blot revealed the expression of p-AMPKα 1 protein was up-regulated, and the expression of p-mTOR and p-S6K was down-regulated after Baohuoside I treatment. Tumorigenesis in nude mice showed that Baohuoside I had an anti-glioma effect in vivo.Conclusion: We propose a natural product, which can inhibit the proliferation, invasion and migration of glioma and may be a valuable anti-tumor candidate. The inhibitory effect of Baohuoside I on the glioma is achieved by inducing the apoptosis of the tumor cells, rather than autophagy. In addition, the pathway to induce cell apoptosis of Baohuoside I is to target the mTOR signal.Keywords: Baohuoside I, mTOR, glioma, apoptosis, proliferation

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