The seluang fish (Rasbora spp.) diet to improve neurotoxicity of endosulfan-induced intrauterine pup's brain through of oxidative mechanism

Abstract

Summary: Epidemiological evidence suggested the relationship between pesticide exposure and increased risk of autism. In autism, there is a change in the production of neurotransmitters and oxidative stress. Seluang fish has high-protein that has not been investigated for its effects on autism. This study aimed to determine the effect of seluang fish on neurotransmitters and oxidative stress in endosulfan-induced rats intrauterine. This study used pup's rat induced by endosulfan intrauterine. The pregnant rats divided into 2 groups were K0 without endosulfan induced and K1 with endosulfan induced. After 4 weeks, 8 pup's rats of K0 and K1 were sacrificed for blood and brain samples, while 8 pup's rats were given a standard diet (P1) and fed a Seluang fish diet (P2) respectively for 4 weeks. Then examined the parameters of serum and brain serotonin, brain H2O2, SOD, and MDA levels. The results showed that K1had increased brain H2O2 and MDA levels, whereas brain and serum serotonin levels and brain SOD decreased significantly compared to K0. Group P1 and P2 showed decreasing brain H2O2 and MDA levels significantly than K1, brain serotonin levels increased significantly than K1, whereas serum serotonin levels were not significantly different (p = 0.483). Group P1 and P2 were not different significantly in the serum and brain serotonin, and brain SOD levels. The brain H2O2 (p < 0.001) and MDA levels (p < 0.001) were different significantly. We concluded that seluang fish diet was able to repair oxidative stress in the brains of rats due to endosulfan induction during pregnancy. Keywords: Autism, Seluang fish, Endosulfan, Neurotransmitter, Oxidative stress