PLoS ONE (Jan 2016)

68Ga and 188Re Starch-Based Microparticles as Theranostic Tool for the Hepatocellular Carcinoma: Radiolabeling and Preliminary In Vivo Rat Studies.

  • Elise Verger,
  • Pierre Drion,
  • Geneviève Meffre,
  • Claire Bernard,
  • Luc Duwez,
  • Nicolas Lepareur,
  • Olivier Couturier,
  • François Hindré,
  • Roland Hustinx,
  • Franck Lacoeuille

DOI
https://doi.org/10.1371/journal.pone.0164626
Journal volume & issue
Vol. 11, no. 10
p. e0164626

Abstract

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This work aims to develop, validate and optimize the radiolabeling of Starch-Based Microparticles (SBMP) by 188Re and 68Ga in the form of ready-to-use radiolabeling kits, the ultimate goal being to obtain a unique theranostic vector for the treatment of Hepatocellular Carcinoma.Optimal labeling conditions and composition of freeze-dried kits were defined by monitoring the radiochemical purity while varying several parameters. In vitro stability studies were carried out, as well as an in vivo biodistribution as a preliminary approach with the intra-arterial injection of 68Ga radiolabeled SBMP into the hepatic artery of DENA-induced rats followed by PET/CT imaging.Kits were optimized for 188Re and 68Ga with high and stable radiochemical purity (>95% and >98% respectively). The in vivo preliminary study was successful with more than 95% of activity found in the liver and mostly in the tumorous part.SBMP are a promising theranostic agent for the Selective Internal Radiation Therapy of Hepatocellular carcinoma.