Construction of a drug, miRNA, and transcription factor regulatory network in membranous nephropathy based on oxidative stress-related genes

Abstract

In this study, oxidative stress-related genes (OSRGs) were used to discover membranous nephropathy (MN) genes, evaluate their biological activities and molecular pathways, and develop regulatory networks for drugs, miRNAs and transcription factors. All 862 differentially expressed genes (DEGs) in the GSE108113 dataset were evaluated using the gene expression matrix. Cross-testing of 437 OSRg in the gene cards revealed 28 DE-OSRG genes. We performed the KEGG and gene ontology enrichment studies. Response to reactive oxygen species, chemical stress, oxidative stress, bound globin-hemoglobin complex, endocytic vesicle lumen, blood microparticles, conjugated globin binding, peroxidase activity and heme binding are the major GO-enriched active DE-OSRGs. As in the KEGG pathway study. After excluding the disconnected genes, 19 DE-OSRGs were introduced into the PPI network, and the top five CDE-OSRGs were found: G6PD, HBB, TP 53, KEAP 1, and PXDN. The ROC curve study shows the CDE-OS.

Keywords

• Membranous nephropathy
• oxidative stress
• regulatory network
• core gene
• miRNA
• transcription factor