Radioprotective Effect of Alk(en)yl Thiosulfates Derived from Allium Vegetables against DNA Damage Caused by X-Ray Irradiation in Cultured Cells: Antiradiation Potential of Onions and Garlic

Hye-Sook Chang; Daiji Endoh; Yushi Ishida; Hiroyuki Takahashi; Shuji Ozawa; Masanobu Hayashi; Akira Yabuki; Osamu Yamato

doi:10.1100/2012/846750

The Scientific World Journal (Jan 2012)

Radioprotective Effect of Alk(en)yl Thiosulfates Derived from Allium Vegetables against DNA Damage Caused by X-Ray Irradiation in Cultured Cells: Antiradiation Potential of Onions and Garlic

Hye-Sook Chang,
Daiji Endoh,
Yushi Ishida,
Hiroyuki Takahashi,
Shuji Ozawa,
Masanobu Hayashi,
Akira Yabuki,
Osamu Yamato

Affiliations

Hye-Sook Chang: Laboratory of Clinical Pathology, Department of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima 890-0065, Japan
Daiji Endoh: Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University, 582 Midorimachi, Bunkyodai, Hokkaido 069-8501, Ebetsu, Japan
Yushi Ishida: Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University, 582 Midorimachi, Bunkyodai, Hokkaido 069-8501, Ebetsu, Japan
Hiroyuki Takahashi: Information Systems Department, Industrial Research Institute, Hokkaido Research Organization, Kita-19 Nishi-11, Hokkaido 060-0819, Sapporo, Japan
Shuji Ozawa: Laboratory of Natural Products Chemistry, Department of Sustainable Agriculture, Rakuno Gakuen University, 582 Midorimachi, Bunkyodai, Hokkaido 069-8501, Ebetsu, Japan
Masanobu Hayashi: Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University, 582 Midorimachi, Bunkyodai, Hokkaido 069-8501, Ebetsu, Japan
Akira Yabuki: Laboratory of Clinical Pathology, Department of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima 890-0065, Japan
Osamu Yamato: Laboratory of Clinical Pathology, Department of Veterinary Medicine, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima 890-0065, Japan

DOI: https://doi.org/10.1100/2012/846750
Journal volume & issue: Vol. 2012

Abstract

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To evaluate a radioprotective effect of sodium n-propyl thiosulfate (NPTS) and sodium 2-propenyl thiosulfate (2PTS) derived from onions and garlic, respectively, rat hepatoma H4IIE cells and mouse lymphoma L5178Y cells were preincubated with each of these compounds for 48 hours at 37°C before receiving 10 Gy of X-ray irradiation. Cell damage caused by the irradiation was quantified as comet tail moment, which represents the degree of DNA damage. X-ray-induced DNA damage was significantly decreased in both H4IIE and L5178Y cells by micromolar concentrations of NPTS and 2PTS compared with the control without the compounds. The protective effect was more potent with 2PTS than NPTS. Onions and garlic have antiradiation potential.

Published in The Scientific World Journal

ISSN: 2356-6140 (Print); 1537-744X (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Technology; Medicine; Science
Website: https://onlinelibrary.wiley.com/journal/8086

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