Anti-Inflammatory Cembrane-Type Diterpenoids and Prostaglandins from Soft Coral <i>Lobophytum sarcophytoides</i>
Hongjie Shen,
Xiaowan Liu,
Minghua Jiang,
Guangyuan Luo,
Zhenger Wu,
Bin Chen,
Jing Li,
Lan Liu,
Senhua Chen
Affiliations
Hongjie Shen
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China
Xiaowan Liu
Shenzhen Key Laboratory for the Sustainable Use of Marine Biodiversity, Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China
Minghua Jiang
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China
Guangyuan Luo
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China
Zhenger Wu
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China
Bin Chen
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China
Jing Li
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China
Lan Liu
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China
Senhua Chen
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China
Two new cembrane-type diterpenoids, lobophytins A (1) and B (3), and four new prostaglandins, (5E)-PGB2 (10), (5E)-13,14-dihydro-PGB2 (11), 13,14-dihydro-PGB2 (12) and 13,14-dihydro-PGB2-Me (13), together with ten known compounds were isolated from the soft coral Lobophytum sarcophytoides. The structures of these new secondary metabolites were identified by high resolution mass spectrometry (HR-ESIMS), nuclear magnetic resonance (NMR) and electron circular dichroism (ECD) analyses, as well as the modified Mosher’s method. Compounds 6, 7, 9, 10, 12, 13, 15 and 16 showed potential anti-inflammatory activity by inhibiting the production of nitric oxide (NO) in RAW264.7 cells that were activated by lipopolysaccharide, with IC50 values ranging from 7.1 to 32.1 μM and were better than the positive control indomethacin, IC50 = 39.8 μM.
