Genes polymorphism of proteins – regulators – of the inflammation at atherosclerosis complicated with development of myocardium infarct

Abstract

Promoter polymorphism TNFC-863A; TNFG-308A; TNFG-238A; IL-1 β C-511T; IL-1 β T-31C; IL4C-590T; IL-6G-174C; IL10А-1082G; IL10C-590A; MMP-2C-1306T; MMP-9C-1562 T; HSP-70-2 A-1267G and HSP-70-HOM C-2437T of genes was investigated with the purpose of research association their genotypes with development of myocardium infarct (MI) at different age of means. For genotyping we used a restriction fragment length polymorphism (RFLP) method of PCR pro ducts (RFLP-analysis) by specific primers. The amplification products were hydrolysis and restriction fragments were analysis. We are investigation 223 patients and 95 healthy men. It Is shown, that practically in each polymorphic site of the investigated genes come to light genotypes, in a various degree associated with development MI. Frequently these genetic risk factors was cooperate with classical risk factors of atherosclerosis development: smoking, age, weight index of the body, arterial pres sure. Complex analysis a lot of genotypes polymorphic sites of genes IL-1β – IL-4- IL-6-IL-10 – TNF-A are show higher parameters of relation risk (OR). Polymorphism genes research of proteins which participating in regulation of inflammation activity is independent value in creation of complex genetic risk factors of development of an atherosclerosis and its vascular complications.

Keywords

• an atherosclerosis
• an inflammation
• myocardium infarct
• cytokine genes
• metalloproteinase genes
• heat shock protein genes