Exploration of the relationship between tumor-infiltrating lymphocyte score and histological grade in breast cancer
Deyong Kang,
Chuan Wang,
Zhonghua Han,
Liqin Zheng,
Wenhui Guo,
Fangmeng Fu,
Lida Qiu,
Xiahui Han,
Jiajia He,
Lianhuang Li,
Jianxin Chen
Affiliations
Deyong Kang
Department of Pathology, Fujian Medical University Union Hospital
Chuan Wang
Breast Surgery Ward, Department of General Surgery, Fujian Medical University Union Hospital
Zhonghua Han
Breast Surgery Ward, Department of General Surgery, Fujian Medical University Union Hospital
Liqin Zheng
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, College of Photonic and Electronic Engineering, Fujian Normal University
Wenhui Guo
Breast Surgery Ward, Department of General Surgery, Fujian Medical University Union Hospital
Fangmeng Fu
Breast Surgery Ward, Department of General Surgery, Fujian Medical University Union Hospital
Lida Qiu
College of Physics and Electronic Information Engineering, Minjiang University
Xiahui Han
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, College of Photonic and Electronic Engineering, Fujian Normal University
Jiajia He
School of Science, Jimei University
Lianhuang Li
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, College of Photonic and Electronic Engineering, Fujian Normal University
Jianxin Chen
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, College of Photonic and Electronic Engineering, Fujian Normal University
Abstract Background The histological grade is an important factor in the prognosis of invasive breast cancer and is vital to accurately identify the histological grade and reclassify of Grade2 status in breast cancer patients. Methods In this study, data were collected from 556 invasive breast cancer patients, and then randomly divided into training cohort (n = 335) and validation cohort (n = 221). All patients were divided into actual low risk group (Grade1) and high risk group (Grade2/3) based on traditional histological grade, and tumor-infiltrating lymphocyte score (TILs-score) obtained from multiphoton images, and the TILs assessment method proposed by International Immuno-Oncology Biomarker Working Group (TILs-WG) were also used to differentiate between high risk group and low risk group of histological grade in patients with invasive breast cancer. Furthermore, TILs-score was used to reclassify Grade2 (G2) into G2 /Low risk and G2/High risk. The coefficients for each TILs in the training cohort were retrieved using ridge regression and TILs-score was created based on the coefficients of the three kinds of TILs. Results Statistical analysis shows that TILs-score is significantly correlated with histological grade, and is an independent predictor of histological grade (odds ratio [OR], 2.548; 95%CI, 1.648–3.941; P 0.05 in the univariate analysis). Moreover, the risk of G2/High risk group is higher than that of G2/Low risk group, and the survival rate of patients with G2/Low risk is similar to that of Grade1, while the survival rate of patients with G2/High risk is even worse than that of patients with G3. Conclusion Our results suggest that TILs-score can be used to predict the histological grade of breast cancer and potentially to guide the therapeutic management of breast cancer patients.
