Molecular characterization of multidrug-resistance in Gram-negative bacteria from the Peshawar teaching hospital, Pakistan

A. Masseron; L. Poirel; B. Jamil Ali; M.A. Syed; P. Nordmann

New Microbes and New Infections (Nov 2019)

Molecular characterization of multidrug-resistance in Gram-negative bacteria from the Peshawar teaching hospital, Pakistan

A. Masseron,
L. Poirel,
B. Jamil Ali,
M.A. Syed,
P. Nordmann

Affiliations

A. Masseron: Medical and Molecular Microbiology, Section of Medicine, Faculty of Science and Medicine, Switzerland
L. Poirel: Medical and Molecular Microbiology, Section of Medicine, Faculty of Science and Medicine, Switzerland; INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland; Swiss National Reference Centre for Emerging Antibiotic Resistance, University of Fribourg, Switzerland; Corresponding author: L. Poirel, University of Fribourg, Fribourg, Switzerland.
B. Jamil Ali: Section of Infectious Diseases, Department of Medicine, The Aga Khan University, Karashi, Pakistan
M.A. Syed: Infectious Diseases Research Group, Department of Microbiology, University of Haripur, Khyber Pakhtunkhwa, Pakistan
P. Nordmann: Medical and Molecular Microbiology, Section of Medicine, Faculty of Science and Medicine, Switzerland; INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland; Swiss National Reference Centre for Emerging Antibiotic Resistance, University of Fribourg, Switzerland; Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland

Journal volume & issue: Vol. 32

Abstract

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Extended-spectrum β-lactamases, carbapenemases, 16S rRNA methylases conferring pan-drug aminoglycoside resistance and colistin resistance were investigated among Gram-negative bacteria recovered from clinical samples (infections) from 200 individuals hospitalized at the Khyber Teaching Hospital of Peshawar, north Pakistan, from December 2017 to March 2018. Out of 65 isolates recovered, 19% were carbapenem resistant and 16% carried a blaNDM-1 gene, confirming the widespread distribution of NDM producers in this country. The association of the NDM carbapenem-resistance determinant, together with the extended-spectrum β-lactamase CTX-M-15 and 16S rRNA methylases, was frequent, explaining the multidrug-resistance pattern observed. All isolates remained susceptible to colistin. Keywords: Carbapenemases, Extended spectrum β-lactamase, Pakistan, ST 215, ST 231, 16S rRNA methylases

Published in New Microbes and New Infections

ISSN: 2052-2975 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases
Website: https://www.sciencedirect.com/journal/new-microbes-and-new-infections

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