Journal of Cardio-Thoracic Medicine (Dec 2022)

Age-Specific Reference Intervals for Routine Laboratory Biochemical Tests in The Iranian Adolescent Girls

  • Mehran Pashirzad,
  • Hamideh Ghazizadeh,
  • Mohsen Seddigh Shamsi,
  • Mahdiyeh Yaghooti-Khorasani,
  • Reza Assaran Darban,
  • Atieh Kamel Khodabandeh,
  • Ensieh Akbarpour Bahre,
  • Maryam Mohammadi Bajgiran,
  • Amin Mansouri,
  • Reza Javid Dash Bayyaz,
  • Parisa Asadian,
  • Khashayar Khanizadeh,
  • Hale Akhtari,
  • Melika Hadizadeh,
  • Arvin Babaei,
  • Sayyed Saeid Khayatzadeh,
  • Gordon A. Ferns,
  • Habibollah Esmaily,
  • Majid Ghayour-Mobarhan

DOI
https://doi.org/10.22038/jctm.2022.67858.1398
Journal volume & issue
Vol. 10, no. 4
pp. 1052 – 1064

Abstract

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Introduction: For interpretation of the most common biochemical markers reference intervals (RIs) are widely considered and reported. Therefore, in this study, we aimed to determine age-specific RIs for some biochemical tests by removing and/or limiting critical gaps including sex, age group, geographical region, measurement device, and type of kits and reagents for routine laboratory biochemical variables in healthy adolescent girls aged 12-19 years.Materials and Methods: In this cross-sectional study a total of 1026 adolescent girl was recruited. According to the study aim, the levels of serum for some routine biochemical tests were measured on a BT-3000 auto-analyzer. On the basis of the non-parametric rank method and by use of CLSI Ep28-A3 guidelines, we determined age-specific RIs, including confidence interval 90%, under specific exclusion criteria.Results: Age partitioning was required for none of the upper and lower limits of biochemical markers. All Serum biochemical tests concentration remained relatively constant throughout the age range. Conclusion: By removing and/or limiting critical gaps involved in reference values of biochemical tests, it has provided an important understanding of metabolic processes and it has facilitated clinical application to track different diseases better

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