Frontiers in Molecular Biosciences (Oct 2021)

Comprehensive Analysis of Aquaporin Superfamily in Lung Adenocarcinoma

  • Guofu Lin,
  • Guofu Lin,
  • Guofu Lin,
  • Luyang Chen,
  • Luyang Chen,
  • Luyang Chen,
  • Lanlan Lin,
  • Lanlan Lin,
  • Lanlan Lin,
  • Hai Lin,
  • Hai Lin,
  • Hai Lin,
  • Zhifeng Guo,
  • Zhifeng Guo,
  • Zhifeng Guo,
  • Yingxuan Xu,
  • Yingxuan Xu,
  • Yingxuan Xu,
  • Chanchan Hu,
  • Jinglan Fu,
  • Jinglan Fu,
  • Qinhui Lin,
  • Qinhui Lin,
  • Wenhan Chen,
  • Wenhan Chen,
  • Wenhan Chen,
  • Yiming Zeng,
  • Yiming Zeng,
  • Yuan Xu,
  • Yuan Xu

DOI
https://doi.org/10.3389/fmolb.2021.736367
Journal volume & issue
Vol. 8

Abstract

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Background: Lung adenocarcinoma (LUAD) is the most predomintnt lung cancer subtype with increasing morbidity and mortality. Previous studies have shown that aquaporin (AQP) family genes were correlated with tumor progression and metastasis in several kinds of malignancies. However, their biological behaviors and prognostic values in LUAD have not been comprehensively elucidated.Methods: RNA sequencing and real-time reverse transcription PCR (RT-PCR) were used to assess AQP1/3/4/5 gene expressions in LUAD patients using GEPIA and UALCAN databases. And then Kaplan–Meier analysis, cBioPortal, Metascape, GeneMANIA, TISIDB, and TIMER were utilized to determine the prognostic value, mutation frequency, and immune cell infiltration of AQP family members in LUAD.Results: We found that AQP3 expression was significantly elevated and AQP1 expression was markedly reduced in LUAD patients, whereas the expression levels of AQP4 and AQP5 exhibited no significant changes. The Kaplan–Meier survival analysis indicated that the higher expressions of AQP1/4/5 were related to longer overall survival (OS). Of interest, AQP3 was significantly correlated with the clinical tumor stage and lower AQP3 expression showed favorable prognosis in stage I LUAD patients, which indicated that AQP3 may be a potential prognostic biomarker for patients. Through functional enrichment analysis, the functions of these four AQPs genes were mainly involved in the passive transport by aquaporins, water homeostasis, and protein tetramerization. Moreover, AQP1/3/4/5 expression was strongly associated with tumor-infiltrating lymphocytes (TILs) in LUAD.Conclusion: AQP3 can be used as a prognosis and survival biomarker for stage I LUAD. These findings may provide novel insights into developing molecular targeted therapies in LUAD.

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