Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots

Chun-Ting Kuo; Alison M. Thompson; Maria Elena Gallina; Fangmao Ye; Eleanor S. Johnson; Wei Sun; Mengxia Zhao; Jiangbo Yu; I-Che Wu; Bryant Fujimoto; Christopher C. DuFort; Markus A. Carlson; Sunil R. Hingorani; Amy L. Paguirigan; Jerald P. Radich; Daniel T. Chiu

doi:10.1038/ncomms11468

Nature Communications (Apr 2016)

Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots

Chun-Ting Kuo,
Alison M. Thompson,
Maria Elena Gallina,
Fangmao Ye,
Eleanor S. Johnson,
Wei Sun,
Mengxia Zhao,
Jiangbo Yu,
I-Che Wu,
Bryant Fujimoto,
Christopher C. DuFort,
Markus A. Carlson,
Sunil R. Hingorani,
Amy L. Paguirigan,
Jerald P. Radich,
Daniel T. Chiu

Affiliations

Chun-Ting Kuo: Department of Chemistry, University of Washington
Alison M. Thompson: Department of Chemistry, University of Washington
Maria Elena Gallina: Department of Chemistry, University of Washington
Fangmao Ye: Department of Chemistry, University of Washington
Eleanor S. Johnson: Department of Chemistry, University of Washington
Wei Sun: Department of Chemistry, University of Washington
Mengxia Zhao: Department of Chemistry, University of Washington
Jiangbo Yu: Department of Chemistry, University of Washington
I-Che Wu: Department of Chemistry, University of Washington
Bryant Fujimoto: Department of Chemistry, University of Washington
Christopher C. DuFort: Clinical Research Division, Fred Hutchinson Cancer Research Center
Markus A. Carlson: Clinical Research Division, Fred Hutchinson Cancer Research Center
Sunil R. Hingorani: Clinical Research Division, Fred Hutchinson Cancer Research Center
Amy L. Paguirigan: Clinical Research Division, Fred Hutchinson Cancer Research Center
Jerald P. Radich: Clinical Research Division, Fred Hutchinson Cancer Research Center
Daniel T. Chiu: Department of Chemistry, University of Washington

DOI: https://doi.org/10.1038/ncomms11468
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 11

Abstract

Read online

Isolation of individual cells from mixed populations is desirable for many biomedical applications. Here the authors use photoswitchable Pdots to allow 'optical painting', where cells of interest are marked based on their visual characteristics, and can then be isolated by fluorescence activated cell sorting.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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