The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells
Hi Chul Kim,
Gi-Hwan Kim,
David Shum,
Ssang-Goo Cho,
Eun Ju Lee,
Yong-Jun Kwon
Affiliations
Hi Chul Kim
Institut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea; Department of Animal Biotechnology (BK21), Animal Resources Research Center, Konkuk University, Seoul 143-702, Republic of Korea
Gi-Hwan Kim
Biomedical Research Institute & IRICT, Seoul National University Hospital, Seoul, Republic of Korea
David Shum
Institut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea
Ssang-Goo Cho
Department of Animal Biotechnology (BK21), Animal Resources Research Center, Konkuk University, Seoul 143-702, Republic of Korea
Eun Ju Lee
Biomedical Research Institute & IRICT, Seoul National University Hospital, Seoul, Republic of Korea; Correspondence to: Biomedical Research Institute and IRICT, Center for Cell & Gene Therapy, Seoul National University Hospital, 101 Daehak ro, jongro gu, Seoul 110-744, Republic of Korea. Tel.: +82 2 2072 1707; fax: +82 2 742 8965.
Yong-Jun Kwon
Institut Pasteur Korea, IP-Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-400, Republic of Korea; Corresponding author. Current address: Ksilink, 16 Rue d’Ankara, Strasbourg 67000, France.
Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, “Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells” (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection.
