The links among Enterocytozoon hepatopenaei infection, growth retardation and intestinal microbiota in different sized shrimp Penaeus vannamei

Abstract

Increasing evidences have showed that a tight link among pathogen infection, physiological indicators and intestinal microbiota. To investigate effects of Enterocytozoon hepatopenaei (EHP) infection to growth retardation and intestinal microbiota, different sized Pacific white shrimp (Penaeus vannamei) infecting with EHP were studied. The shrimp with big, medium and small sized groups were from same batch of postlarvae and reared in one pond under the same diet and environmental conditions. Number of EHP copies revealed that the more EHP copies the smaller size of shrimp individuals. Denaturing gradient gel electrophoresis (DGGE) profiles showed intestinal bacterial patterns of small sized shrimp were similar to the middle sized shrimp, rather than the big sized shrimp. It implied that intestinal microbiota were influenced by severity of EHP infection and suggested the different sizes of shrimp were due to the successive infection period of EHP. Among the different sized groups, the relative abundance of Vibrio of small group was the highest, suggesting EHP infection could result in higher susceptibility to pathogen invasion. Our findings provided the evidence intestinal bacterial patterns altered by infection of EHP, the more EHP copies the smaller size of shrimp, and different sized individuals due to successive infection period of EHP.

Keywords

• Enterocytozoon hepatopenaei
• Intestinal microbiota
• DGGE
• Growth retardation