Evaluation of Fibrin-Based Interpenetrating Polymer Networks as Potential Biomaterials for Tissue Engineering
Olfat Gsib,
Jean-Luc Duval,
Mathieu Goczkowski,
Marie Deneufchatel,
Odile Fichet,
Véronique Larreta-Garde,
Sidi Ahmed Bencherif,
Christophe Egles
Affiliations
Olfat Gsib
Laboratoire de BioMécanique et de BioIngénierie (BMBI) UMR CNRS 7388, Sorbonne Universités, Université de Technologie of Compiègne (UTC), 60200 Compiègne, France
Jean-Luc Duval
Laboratoire de BioMécanique et de BioIngénierie (BMBI) UMR CNRS 7388, Sorbonne Universités, Université de Technologie of Compiègne (UTC), 60200 Compiègne, France
Mathieu Goczkowski
Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (Errmece), Institut des Matériaux, Université de Cergy-Pontoise, 95000 Cergy-Pontoise, France
Marie Deneufchatel
Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (Errmece), Institut des Matériaux, Université de Cergy-Pontoise, 95000 Cergy-Pontoise, France
Odile Fichet
Laboratoire de Physicochimie des Polymères et des Interfaces (LPPI), Institut des Matériaux, Université de Cergy-Pontoise, 95000 Cergy-Pontoise, France
Véronique Larreta-Garde
Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (Errmece), Institut des Matériaux, Université de Cergy-Pontoise, 95000 Cergy-Pontoise, France
Sidi Ahmed Bencherif
Laboratoire de BioMécanique et de BioIngénierie (BMBI) UMR CNRS 7388, Sorbonne Universités, Université de Technologie of Compiègne (UTC), 60200 Compiègne, France
Christophe Egles
Laboratoire de BioMécanique et de BioIngénierie (BMBI) UMR CNRS 7388, Sorbonne Universités, Université de Technologie of Compiègne (UTC), 60200 Compiègne, France
Interpenetrating polymer networks (IPNs) have gained great attention for a number of biomedical applications due to their improved properties compared to individual components alone. In this study, we investigated the capacity of newly-developed naturally-derived IPNs as potential biomaterials for tissue engineering. These IPNs combine the biologic properties of a fibrous fibrin network polymerized at the nanoscale and the mechanical stability of polyethylene oxide (PEO). First, we assessed their cytotoxicity in vitro on L929 fibroblasts. We further evaluated their biocompatibility ex vivo with a chick embryo organotypic culture model. Subcutaneous implantations of the matrices were subsequently conducted on nude mice to investigate their biocompatibility in vivo. Our preliminary data highlighted that our biomaterials were non-cytotoxic (viability above 90%). The organotypic culture showed that the IPN matrices induced higher cell adhesion (across all the explanted organ tissues) and migration (skin, intestine) than the control groups, suggesting the advantages of using a biomimetic, yet mechanically-reinforced IPN-based matrix. We observed no major inflammatory response up to 12 weeks post implantation. All together, these data suggest that these fibrin-based IPNs are promising biomaterials for tissue engineering.