Brain damage led to decrease of nitric oxide content in the hippocampus of rats: Investigation by EPR spectroscopy

Abstract

Direct measurements of the content of nitric oxide (NO) by the method of spectroscopy of electron paramagnetic resonance were performed. It was found that NO production in hippocampus tissues decreased to 46% after 5 h of ischemic stroke as compared to the control group and remained at the same level after 72 h. Following the hemorrhagic stroke, the level of NO production in the hippocampus decreased to 32% after 5 h of hemorrhagic stroke of the control level. After 72 hours of hemorrhagic stroke NO production was higher than after 5 h and amounted to 48% of the level of intact animals.

Keywords

• nitric oxide
• ischemic brain stroke
• hemorrhagic brain stroke
• hippocampus
• electron paramagnetic resonance