Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring (Jan 2021)
The global Alzheimer's Association round robin study on plasma amyloid β methods
- Josef Pannee,
- Leslie M. Shaw,
- Magdalena Korecka,
- Teresa Waligorska,
- Charlotte E. Teunissen,
- Erik Stoops,
- Hugo M. J. Vanderstichele,
- Kimberley Mauroo,
- Inge M. W. Verberk,
- Ashvini Keshavan,
- Pedro Pesini,
- Leticia Sarasa,
- Maria Pascual‐Lucas,
- Noelia Fandos,
- José‐Antonio Allué,
- Erik Portelius,
- Ulf Andreasson,
- Ritsuko Yoda,
- Akinori Nakamura,
- Naoki Kaneko,
- Shieh‐Yueh Yang,
- Huei‐Chun Liu,
- Stefan Palme,
- Tobias Bittner,
- Kwasi G. Mawuenyega,
- Vitaliy Ovod,
- James Bollinger,
- Randall J. Bateman,
- Yan Li,
- Jeffrey L. Dage,
- Erik Stomrud,
- Oskar Hansson,
- Jonathan M. Schott,
- Kaj Blennow,
- Henrik Zetterberg
Affiliations
- Josef Pannee
- Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology The Sahlgrenska Academy, University of Gothenburg Mölndal Sweden
- Leslie M. Shaw
- Perelman School of Medicine Department of Pathology and Laboratory Medicine University of Pennsylvania Philadelphia Pennsylvania USA
- Magdalena Korecka
- Perelman School of Medicine Department of Pathology and Laboratory Medicine University of Pennsylvania Philadelphia Pennsylvania USA
- Teresa Waligorska
- Perelman School of Medicine Department of Pathology and Laboratory Medicine University of Pennsylvania Philadelphia Pennsylvania USA
- Charlotte E. Teunissen
- Neurochemistry Laboratory Department of Clinical Chemistry Amsterdam Neuroscience Amsterdam UMC Vrije Universiteit Amsterdam Amsterdam the Netherlands
- Erik Stoops
- ADx NeuroSciences Ghent Belgium
- Hugo M. J. Vanderstichele
- Biomarkable Ghent Belgium
- Kimberley Mauroo
- ADx NeuroSciences Ghent Belgium
- Inge M. W. Verberk
- Neurochemistry Laboratory Department of Clinical Chemistry Amsterdam Neuroscience Amsterdam UMC Vrije Universiteit Amsterdam Amsterdam the Netherlands
- Ashvini Keshavan
- Dementia Research Centre UCL Queen Square Institute of Neurology London UK
- Pedro Pesini
- Araclon Biotech Zaragoza Spain
- Leticia Sarasa
- Araclon Biotech Zaragoza Spain
- Maria Pascual‐Lucas
- Araclon Biotech Zaragoza Spain
- Noelia Fandos
- Araclon Biotech Zaragoza Spain
- José‐Antonio Allué
- Araclon Biotech Zaragoza Spain
- Erik Portelius
- Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology The Sahlgrenska Academy, University of Gothenburg Mölndal Sweden
- Ulf Andreasson
- Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology The Sahlgrenska Academy, University of Gothenburg Mölndal Sweden
- Ritsuko Yoda
- Koichi Tanaka Mass Spectrometry Research Laboratory Shimadzu Corporation Kyoto Japan
- Akinori Nakamura
- Department of Biomarker Research National Center for Geriatrics and Gerontology Obu Aichi Japan
- Naoki Kaneko
- Koichi Tanaka Mass Spectrometry Research Laboratory Shimadzu Corporation Kyoto Japan
- Shieh‐Yueh Yang
- MagQu Co., Ltd. New Taipei City Taiwan
- Huei‐Chun Liu
- MagQu Co., Ltd. New Taipei City Taiwan
- Stefan Palme
- Roche Diagnostics GmbH Penzberg Germany
- Tobias Bittner
- F. Hoffmann‐La Roche Ltd Basel Switzerland
- Kwasi G. Mawuenyega
- Department of Neurology Washington University School of Medicine St. Louis Missouri USA
- Vitaliy Ovod
- Department of Neurology Washington University School of Medicine St. Louis Missouri USA
- James Bollinger
- Department of Neurology Washington University School of Medicine St. Louis Missouri USA
- Randall J. Bateman
- Department of Neurology Washington University School of Medicine St. Louis Missouri USA
- Yan Li
- Department of Neurology Washington University School of Medicine St. Louis Missouri USA
- Jeffrey L. Dage
- Eli Lilly and Company Indianapolis Indiana USA
- Erik Stomrud
- Clinical Memory Research Unit Faculty of Medicine Lund University Lund Sweden
- Oskar Hansson
- Clinical Memory Research Unit Faculty of Medicine Lund University Lund Sweden
- Jonathan M. Schott
- Dementia Research Centre UCL Queen Square Institute of Neurology London UK
- Kaj Blennow
- Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology The Sahlgrenska Academy, University of Gothenburg Mölndal Sweden
- Henrik Zetterberg
- Department of Psychiatry and Neurochemistry Institute of Neuroscience and Physiology The Sahlgrenska Academy, University of Gothenburg Mölndal Sweden
- DOI
- https://doi.org/10.1002/dad2.12242
- Journal volume & issue
-
Vol. 13,
no. 1
pp. n/a – n/a
Abstract
Abstract Introduction Blood‐based assays to measure brain amyloid beta (Aβ) deposition are an attractive alternative to the cerebrospinal fluid (CSF)–based assays currently used in clinical settings. In this study, we examined different blood‐based assays to measure Aβ and how they compare among centers and assays. Methods Aliquots from 81 plasma samples were distributed to 10 participating centers. Seven immunological assays and four mass‐spectrometric methods were used to measure plasma Aβ concentrations. Results Correlations were weak for Aβ42 while Aβ40 correlations were stronger. The ratio Aβ42/Aβ40 did not improve the correlations and showed weak correlations. Discussion The poor correlations for Aβ42 in plasma might have several potential explanations, such as the high levels of plasma proteins (compared to CSF), sensitivity to pre‐analytical sample handling and specificity, and cross‐reactivity of different antibodies. Different methods might also measure different pools of plasma Aβ42. We, however, hypothesize that greater correlations might be seen in future studies because many of the methods have been refined during completion of this study.
Keywords
