Nature Communications (Jun 2018)

CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

  • Toon Swings,
  • David C. Marciano,
  • Benu Atri,
  • Rachel E. Bosserman,
  • Chen Wang,
  • Marlies Leysen,
  • Camille Bonte,
  • Thomas Schalck,
  • Ian Furey,
  • Bram Van den Bergh,
  • Natalie Verstraeten,
  • Peter J. Christie,
  • Christophe Herman,
  • Olivier Lichtarge,
  • Jan Michiels

DOI
https://doi.org/10.1038/s41467-018-04651-5
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 10

Abstract

Read online

Genome editing requires precise targeting of loci with specific gRNAs. Here the authors introduce CRISPR-FRT, which targets flippase recognition sites, common in bacterial genetic collections, for fast off-the-shelf genome engineering.