HPLC analysis and cell surface receptor binding activities of the crude aqueous and methanolic extract of Sesamum indicum

Abstract

Objective: To identify the possible functional molecules for therapeutic uses by screening the crude aqueous and methanolic extracts derived from sesame seeds (Sesamum indicum) in vitro. Methods: High performance liquid chromatography was used to scan the functional molecules present in the extracts. Results: The crude aqueous extracts showed the possibilities to present caffeine and cetirizine or its derivatives like molecules. On the other hand, the crude methanolic extract may contain Loratadine or its derivatives like molecules. Both type of extracts showed hemagglutination inhibition activities in all types of human blood samples tested. However, they showed stronger binding with AB+ blood group than those of A+ and B+ blood. Conclusions: Sesame seeds may be considered as a functional food.

Keywords

• Sesamum indicum
• Hemagglutination
• High performance liquid chromatography
• Thin-layer chromatography