Nature Communications (Jul 2019)
The generation and use of recombinant extracellular vesicles as biological reference material
- Edward Geeurickx,
- Joeri Tulkens,
- Bert Dhondt,
- Jan Van Deun,
- Lien Lippens,
- Glenn Vergauwen,
- Elisa Heyrman,
- Delphine De Sutter,
- Kris Gevaert,
- Francis Impens,
- Ilkka Miinalainen,
- Pieter-Jan Van Bockstal,
- Thomas De Beer,
- Marca H. M. Wauben,
- Esther N. M. Nolte-‘t-Hoen,
- Katarzyna Bloch,
- Johannes V. Swinnen,
- Edwin van der Pol,
- Rienk Nieuwland,
- Geert Braems,
- Nico Callewaert,
- Pieter Mestdagh,
- Jo Vandesompele,
- Hannelore Denys,
- Sven Eyckerman,
- Olivier De Wever,
- An Hendrix
Affiliations
- Edward Geeurickx
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University
- Joeri Tulkens
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University
- Bert Dhondt
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University
- Jan Van Deun
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University
- Lien Lippens
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University
- Glenn Vergauwen
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University
- Elisa Heyrman
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University
- Delphine De Sutter
- Department of Biomolecular Medicine, Ghent University
- Kris Gevaert
- Cancer Research Institute Ghent
- Francis Impens
- Cancer Research Institute Ghent
- Ilkka Miinalainen
- Biocenter Oulu, University of Oulu
- Pieter-Jan Van Bockstal
- Laboratory of Pharmaceutical Process Analytical Technology, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University
- Thomas De Beer
- Laboratory of Pharmaceutical Process Analytical Technology, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University
- Marca H. M. Wauben
- Department of Biochemistry and Cell Biology, Faculty of Veterinary medicine, Utrecht University
- Esther N. M. Nolte-‘t-Hoen
- Department of Biochemistry and Cell Biology, Faculty of Veterinary medicine, Utrecht University
- Katarzyna Bloch
- Laboratory of Lipid Metabolism and Cancer, Department of Oncology, University of Leuven
- Johannes V. Swinnen
- Laboratory of Lipid Metabolism and Cancer, Department of Oncology, University of Leuven
- Edwin van der Pol
- Laboratory of Experimental Clinical Chemistry, Amsterdam University Medical Center, Amsterdam University
- Rienk Nieuwland
- Laboratory of Experimental Clinical Chemistry, Amsterdam University Medical Center, Amsterdam University
- Geert Braems
- Department of Gynaecology, Ghent University Hospital
- Nico Callewaert
- Cancer Research Institute Ghent
- Pieter Mestdagh
- Cancer Research Institute Ghent
- Jo Vandesompele
- Cancer Research Institute Ghent
- Hannelore Denys
- Cancer Research Institute Ghent
- Sven Eyckerman
- Cancer Research Institute Ghent
- Olivier De Wever
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University
- An Hendrix
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University
- DOI
- https://doi.org/10.1038/s41467-019-11182-0
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 12
Abstract
There is no universal reference material to develop extracellular vesicle (EV) separation methods and carry out calibration and normalization. Here the authors use HIV-derived gag proteins to assemble recombinant fluorescent EV as a trackable reference material resembling the physical and biochemical properties of sample EV.
