Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

Duverney Chaverra-Rodriguez; Vanessa M. Macias; Grant L. Hughes; Sujit Pujhari; Yasutsugu Suzuki; David R. Peterson; Donghun Kim; Sage McKeand; Jason L. Rasgon

doi:10.1038/s41467-018-05425-9

Nature Communications (Aug 2018)

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

Duverney Chaverra-Rodriguez,
Vanessa M. Macias,
Grant L. Hughes,
Sujit Pujhari,
Yasutsugu Suzuki,
David R. Peterson,
Donghun Kim,
Sage McKeand,
Jason L. Rasgon

Affiliations

Duverney Chaverra-Rodriguez: Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State University
Vanessa M. Macias: Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State University
Grant L. Hughes: Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State University
Sujit Pujhari: Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State University
Yasutsugu Suzuki: Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State University
David R. Peterson: Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State University
Donghun Kim: Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State University
Sage McKeand: Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State University
Jason L. Rasgon: Department of Entomology, The Huck Institutes of the Life Sciences, and the Center for infectious Disease Dynamics, The Pennsylvania State University

DOI: https://doi.org/10.1038/s41467-018-05425-9
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 11

Abstract

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Delivery of Cas9 ribonucleoprotein complexes can be done via microinjection into eggs, though this is a technically challenging procedure. Here the authors demonstrate ReMOT Control, delivery of RNPs to the mosquito germline by tagging with peptide derived from yolk protein 1.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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