Bulletin of Faculty of Pharmacy Cairo University (Jun 2013)

Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor

  • Hewaida F. El-Sedawy,
  • Manal M.M. Hussein,
  • Tamer Essam,
  • Ossama M. El-Tayeb,
  • Fatma H.A. Mohammad

DOI
https://doi.org/10.1016/j.bfopcu.2013.02.002
Journal volume & issue
Vol. 51, no. 1
pp. 43 – 48

Abstract

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Optimization of fermentation process conditions using a gene amplified variant of Amycolatopsis mediterranei (NCH) was carried out. The use of aeration level 1.5 vvm increased the yield by 16.6% (from 13.81 to 16.1 g/l) upon controlling the temperature at 28 °C. Adjustment of the aeration level at 1.5 vvm for 3 days then controlling the dissolved oxygen (DO) at 30% saturation further increased the yield to 17.8 g/l. The optimum pH was 6.5 for 3 days then 7 thereafter when a production yield of 16.1 g/l was recorded using an aeration rate of 1.5 vvm. Controlling the pH at constant value (6.5 or 7) all through the fermentation process decreased the yield by 5–21%. Controlling the temperature at 30 °C for 3 days then 28 °C thereafter slightly increased the yield by 5% upon using an aeration rate of 1 vvm while it decreased upon using an aeration rate of 1.5 vvm. Integration of the most optimum conditions increased the production yield by 22% from 13.81 to 17.8 g/l.

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