Frontiers in Cellular and Infection Microbiology (Sep 2020)

The CD133+ Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women

  • Jacqueline María Valverde-Villegas,
  • Mar Naranjo-Gomez,
  • Mar Naranjo-Gomez,
  • Mélusine Durand,
  • David Rutagwera,
  • Anne-Sophie Bedin,
  • Chipepo Kankasa,
  • Ségolène Debiesse,
  • Nicolas Nagot,
  • Nicolas Nagot,
  • Edouard Tuaillon,
  • Edouard Tuaillon,
  • Philippe Van de Perre,
  • Philippe Van de Perre,
  • Jean-Pierre Molès

DOI
https://doi.org/10.3389/fcimb.2020.546189
Journal volume & issue
Vol. 10

Abstract

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Human milk is a significant source of different CD133+ and/or CD34+ stem/progenitor-like cell subsets in healthy women but their cell distribution and percentages in this compartment of HIV-positive women have not been explored. To date, a decrease of CD34+ hematopoietic stem and progenitor cell frequencies in peripheral blood and bone marrow of HIV-positive patients has been reported. Herein, human milk and peripheral blood samples were collected between day 2–15 post-partum from HIV-positive and HIV-negative women, and cells were stained with stem cell markers and analyzed by flow cytometry. We report that the median percentage of CD45+/highCD34−CD133+ cell subset from milk and blood was significantly higher in HIV-positive than in HIV-negative women. The percentage of CD45dimCD34−CD133+ cell subset from blood was significantly higher in HIV-positive than HIV-negative women. Moreover, percentages of CD45dimCD34+, CD45dimCD34+CD133−, and CD45+highCD34+CD133− cell subsets from blood were significantly lower in HIV-positive than HIV-negative women. The CD133+ stem/progenitor-like cell subsets are increased in early human milk and blood of HIV-positive women and are differentially distributed to CD34+ cell subset frequencies which are decreased in blood.

Keywords