Structural insights into mechanism and specificity of the plant protein O-fucosyltransferase SPINDLY

Abstract

Arabidopsis SPINDLY (SPY) is a nucleocytoplasmic protein O-fucosyltransferase. Here, the authors present a crystal structure of Arabidopsis SPY/GDP complex, reveal SPY’s substrate recognition and enzyme mechanism, and provide insights into the glycan donor substrate selection in GT41 proteins.